Genetic diversity of Origanum syriacum L. (Lamiaceae) species through Touch-Up Direct Amplification of Minisatellite-region DNA (TU-DAMD) marker

Origanum syriacum L. (Lamiaceae) is a perennial species with an important role in medicinal and pharmaceutical applications. However, the attention regarding its genetic diversity negligible worldwide. Touch-up direct amplification of minisatellite-region DNA (TU-DAMD) marker has been employed to investigate natural populations, where 37 wild O. genotypes were collected from coastal, central southern regions Syria. TU-DAMD assay resulted 188 total bands, which 152 (80.85%) polymorphic. The bands number ranged between 7 22 average 11.75 bands/primer. Polymorphic 4 19 9.5 polymorphic Moreover, information content (PIC) value 0.051 0.322 0.225. As for index (MI), this 0.308 5.092 2.284. Overall, present study suggests that samples coastal region (Lattakia, Jableh, Banyas Tartous) are genetically distinct ones (Homs, Hama, Damascus, Swedah Qunetra) based on estimated percent disagreement values (PDV) 0.20. Thereby, test successfully discriminate showing high within studied species. In conclusion, could be considered as new tool studying other plant